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KMID : 0545120040140010128
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Journal of Microbiology and Biotechnology
2004 Volume.14 No. 1 p.128 ~ p.133
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Secretion Capacity Limitations of the Sec Pathway in Escherichia coli
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FILIPE MERGULHA¨O
GABRIEL A. MONTEIRO
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Abstract
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The secretion capacity of two E. coli strains (JM109 and AF1000) was evaluated through the expression of two human proinsulin fusion proteins using the translocation signal sequence from Staphylococcal protein A (SPA). Although a 7 to ll-fold difference in the expression levels was attained by the use of different promoters (SPA and malK promoters) and copy-number vectors (700 and 50 copies per cell), the maximum translocation rates for all the systems were around 140,000 amino acids cell-´ min^(-1). Moreover, the secretion capacity was found to be independent of the size of the exiting peptide and its translational rate.
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